D-Aloulose-DEAase Enzyme Immobilization Carrier and Separation of Mixed Sugar Solution

Aloulose is a white solid crystal with no odor, high solubility, and a soft taste. It has a sweetness of 70% of traditional sweetener sucrose, but provides almost no calories. The Maillard reaction with proteins in food, such as egg protein, can improve the gel properties of food, enhance the taste of food, and reduce oxidation during food processing, so it is very suitable for the food industry.

Compared to glucose, alloulose has a lower absorption rate in the human body and can compete with glucose and fructose transporters on the cell membrane surface, thereby reducing the absorption of fructose and glucose in daily diet.

The special physiological properties of alotose make it important for human health, such as enhancing insulin tolerance, inhibiting postprandial blood glucose rise, reducing intra-abdominal fat accumulation, and preventing diabetes.

At present, the production of alloulose is catalyzed by biological enzymes, namely D-tagatose-3-epimerase (DTase), which includes both D-tagatose-3-epimerase (DPEase) and D-alluose-3-epimerase (DAEase).

D-Aloulose-3-Isomerase can effectively isomerize fructose into Aloulose, but most of them have disadvantages such as poor thermal stability, insufficient conversion rate, and low efficiency, which hinder their industrial application. Industrial production may require improving conversion rates and the thermal stability of enzymes.

The immobilization of enzymes has always been an important method for improving their thermal stability.

The enzyme immobilization carrier resin provided by Haipu can immobilize DAEase on ion exchange resin, allowing it to maintain catalytic activity for several months. At the same time, after repeated use, the enzyme activity recovery rate can still be maintained at a high level.

In addition, due to the reversible isomerization reaction of fructose catalyzed by alloketose-3-isomerase, the conversion rate of fructose at equilibrium is only up to 33%. However, alloketose and fructose are two monosaccharides that are isomers of each other, and their physical and chemical properties are almost identical, which poses great difficulties for the separation of alloketose.

Haipu can provide a mature separation and purification technology for aloulose, called Simulated Moving Bed (SMB), which utilizes the difference in adsorption capacity of different substances on chromatographic resins to separate them from each other.

In production, after being catalyzed by immobilized enzymes, the mixed sugar solution is separated using SMB. After simulating a fluidized bed, the purity of aloulose can reach 98.5% (relevant data, for reference only). The simulated fluidized bed achieves continuous production of aloulose and has high production efficiency.