Why Are Hydrophobic Carriers Advantageous for Immobilizing Enzymes?

#Haipu separation and purification immobilized enzyme carrier enzymes are a type of biocatalyst, and the chemical essence of the vast majority of enzymes is protein. Compared with chemical catalysts, enzymes have the advantages of strong specificity, high catalytic efficiency, mild reaction conditions, and controllable activity. However, due to the instability of enzymatic methods, they are highly susceptible to external environmental influences and lose their catalytic activity. In addition, most enzymes are water-soluble, which makes them difficult to separate from substrates and products after catalytic reactions. This not only affects the purity of the products but also prevents the enzymes from being reused, resulting in the inability of enzyme catalysis to achieve ideal economic benefits in industrial applications due to high cost constraints. The emergence of enzyme immobilization technology provides an effective way to overcome the above drawbacks and achieve large-scale applications. As an important branch of enzyme engineering, immobilized enzyme technology is a technique that uses physical and chemical methods to immobilize water-soluble enzymes on specific carriers or restrict enzyme movement within a certain spatial range, while still allowing enzymes to perform their catalytic functions and be reused after the reaction.

Compared with free enzymes, immobilized enzymes not only retain the advantages of water-soluble enzymes, but also have the advantages of reusability, improved activity, simple process, and scalable application. Currently, immobilized enzymes have been widely used in the fields of medicine, food, chemical industry, materials science, environmental protection, energy, proteomics, etc., with very broad development prospects. Characteristics of immobilized enzyme resin carrier: 1) It has high physical and chemical stability; 2) Has low expansibility in high concentration solutions and conventional solvents; 3) Dense cross-linking enhances its mechanical permeability; 4) It has biological affinity and compatibility, which is conducive to the activity of enzymes; 5) There is a high enzyme loading capacity, and the carrier surface can provide multiple active sites, which is conducive to enzyme molecule coupling; 6) Easy to recycle and reuse; Haipu has developed a series of immobilized enzyme carriers (epoxy, hydroxyl, amino, hydrophobic, etc.) with a rich variety of skeleton, pore structure, and binding group types. The products have the characteristics of uniform particle size, high enzyme activity, fast reaction speed, and excellent mechanical strength, which can meet the immobilization needs of different industries and types of enzymes.

By designing in terms of ion strength, pore volume, pore size, and particle size, we provide immobilization solutions for enzyme carriers in fields such as medicine, food, energy, proteomics, etc.