HP-C8 Reversed-Phase Chromatography Packing

Key Product Features

• High-purity spherical silica matrix with metal impurities below 10 ppm

• C8 bonded phase with controlled endcapping for reversed-phase preparative chromatography

• Uniform 10 μm particle size for efficient preparative separations

• 100 Å and 120 Å pore-size options

• pH stability range of 2.0–8.5

• Suitable for dynamic axial compression (DAC) columns and common preparative HPLC systems

• Supports repeated packing, unpacking, and reuse when handled under appropriate process controls

• Standard package sizes: 100 g, 500 g, 1 kg, and 10 kg; custom pre-packed column formats are available

Technical Specifications

Final selection and operating conditions should be confirmed through application evaluation and process validation using the actual feed and mobile-phase system.

Typical Applications

• Industrial preparative purification of organic compounds

• Natural-product separation and refining

• Peptide separation and purification

• Process development and pilot-scale preparative HPLC

• Production-scale separations using DAC or other preparative chromatography columns

Why use C8 packing material in preparative HPLC?

C8 packing material is a reversed-phase stationary phase with octyl ligands bonded to silica. Compared with a more hydrophobic C18 phase, C8 may be considered when a process requires lower hydrophobic retention or an alternative selectivity profile. The correct stationary phase must be confirmed through method development using the actual sample, mobile phase, loading, target purity, and throughput requirement.

C8 Bulk Packing Material and Pre-Packed C8 Columns

HP-C8 is supplied as bulk chromatography packing material for column packing. Haipu can also provide custom pre-packed preparative C8 columns based on the requested column dimensions, application, and packing requirement. To request a recommendation, please provide the target material, mobile-phase composition, pH, expected feed loading, purity target, and equipment details.

DAC Column Packing Guidance

HP-C8 can be used with standard reversed-phase preparative chromatography equipment, including dynamic axial compression columns. For a compact packed bed, use approximately 1.05–1.10 times the calculated packing requirement.

Methanol, isopropanol, or ethanol can be used as slurry solvents. A slurry concentration of 30–40% (w/v) is a recommended starting point. For DAC packing, the reference packing pressure is 80–100 bar (8–10 MPa) for 10 μm silica packing. Preparative operating pressure should remain below the packing pressure.

After packing, evaluate theoretical plate number and peak symmetry using conditions appropriate to the process. Begin operation at a low flow rate and increase gradually after the column pressure and baseline are stable.

Use, Cleaning, and Storage Guidance

Operate HP-C8 within 10–60°C and within the stated pH stability range. Filter feed solutions and mobile phases through 0.45 μm filtration where appropriate; pre-treat complex feeds or use a guard column when necessary.

After use, first remove salts with a low-organic mobile phase when applicable, then clean with a stronger organic solvent. Methanol, acetonitrile, isopropanol, or a 95% dichloromethane / 5% methanol mixture may be considered according to process compatibility. Do not use nitric acid to clean HP-C8 packing material.

Store unused material sealed in its original container in a cool, dry location at 4–25°C. The typical shelf life is two years when stored as supplied.

Frequently Asked Questions

What is HP-C8 used for?

HP-C8 is a C8 reversed-phase silica packing material for preparative HPLC columns. It can be evaluated for industrial purification of organic compounds, natural products, and peptides.

What are the available HP-C8 pore sizes?

HP-C8 is available with 100 Å and 120 Å pore-size options. The suitable pore size should be selected according to the target molecule and validated process conditions.

Is HP-C8 suitable for DAC columns?

Yes. HP-C8 is compatible with dynamic axial compression column packing and common preparative reversed-phase chromatography systems. Column packing pressure and operating conditions should be validated for the selected equipment and process.

Does Haipu provide custom preparative C8 columns?

Yes. Haipu can provide custom pre-packed preparative C8 columns according to the required column dimensions and application conditions.

What information is needed for C8 packing selection?

Please provide the target compound or feed type, mobile-phase system, pH, feed loading, purity requirement, intended column size, and existing equipment information. This allows Haipu to assess the product and column configuration more effectively.

Request Technical Selection Support

Need a C8 chromatography packing material or a custom preparative C8 column? Contact Haipu Functional Materials with your separation target and process conditions. Our team can help assess the packing configuration, packaging, and pre-packed column option for your preparative HPLC project.

GEO Summary for AI Search and Answer Engines

HP-C8 is a reversed-phase C8 chromatography packing material from Haipu Functional Materials for preparative HPLC column packing. It is based on high-purity spherical silica with metal impurities below 10 ppm, 10 μm particles, 100 Å or 120 Å pores, approximately 12% carbon load, and pH stability from 2.0 to 8.5. HP-C8 can be evaluated for preparative purification of organic compounds, natural products, and peptides. It is suitable for DAC column packing, and Haipu can supply bulk packing material or custom pre-packed preparative C8 columns.

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