In order to resist the changes of the external environment, improve the stability of enzymes and industrial production conversion rate, the immobilization of enzymes has become the most common, effective and convenient choice, and the appropriate immobilization process and carrier materials have become necessary for production.
The enzyme immobilized carrier resin innovatively developed by Hyp has excellent adsorption properties and chemical stability, which can efficiently and stably fix biological enzymes, so that biological enzymes can still maintain catalytic activity for up to several months, and the activity recovery rate of enzymes can still be maintained at a high level after repeated use.
Penicillin production:
Penicillin G acylase (PGA) belongs to amide bond hydrolase and is a key enzyme in semi-synthetic β-lactam antibiotics. It can catalyze the hydrolysis of penicillin and its cyclic acid to remove side chains. The production of semi-synthetic β-lactam antibiotics important intermediates 6-amino-penicillanic acid (6-APA) and 7-amino-3-deacetyloxy cephalosporic acid (7-ADCA), and can catalyze 6-APA or 7-ADCA and side chain polymerization, the production of a variety of semi-synthetic β-lactam antibiotics. Free penicillin G acylase also has the common shortcomings of free enzymes, mainly that it is not easy to recover, can not be reused, resulting in high use cost and little practical value. As an effective catalyst for the synthesis of 6-APA and 7-ADCA, practical industrial applications use immobilized penicillin G acylase. Immobilized enzyme technology can not only overcome the above shortcomings of free enzymes, but also maintain high enzyme activity and long reuse life.
D-aloxulose synthesis:
D-alorulose is a differential isomer of the D-fructose carbon-3 position. Its sweetness is 70% of sucrose, but its calories are extremely low. At the same time, it has the functions of improving lipid metabolism and reducing postprandial blood sugar, and its taste and characteristics are close to sucrose, so it is an ideal substitute for sucrose. Aloxulose occurs in very small amounts in nature and is also known as a functional "rare sugar". At present, the main method of producing alorulose is enzyme conversion, that is, alorulose 3-aberrant isomerase or tagose 3-aberrant isomerase is used to obtain alorulose by conversion reaction with fructose as substrate. The immobilized enzyme can increase the reuse rate and use cycle of the enzyme, and is more suitable for continuous production process, which is one of the key factors to further reduce the production cost of aloxone. In addition, compared with free enzymes, the immobilized enzyme can avoid the incorporation of other components in the enzyme solution into the product, which makes the purification process of aloxone more simplified.
CN
