L-Ribose Isomerase Immobilization Carrier

Abstract: Haipu isomerase immobilized carrier refers to the loading of biologically active enzymes onto resins to form immobilized enzymes. Compared with free enzymes, immobilized enzymes have the advantages of high stability, easy recovery, easy separation from reaction systems, multiple reusability, and low cost.

#L-riboisomerase immobilized carrier ribose is a five carbon sugar that is a component of various ribonucleotides, nucleotide coenzymes, ATP, and NADP. It is closely related to biological genetics and plays an important regulatory role in the physiological activity of organisms.

Ribose in nature mainly exists in the form of D-ribose, which exists in plant and animal cells in the form of furanose. D-ribose is also a component of various vitamins, coenzymes, and certain antibiotics such as neomycin A, B, and C; L-ribose is a chiral enantiomer opposite to D-ribose, which does not exist in nature or in living organisms, and is an extremely expensive rare sugar.

Pure L-ribose is a white crystal or white crystal powder with a sweet taste; Soluble in water, ethanol, and methanol, insoluble in ether, benzene, and acetone. L-ribose has excellent anti-tumor and antiviral abilities with minimal toxic side effects on normal cells.

L-ribose is an important intermediate for drug synthesis, which can be used to synthesize nucleoside compounds with antiviral activity. It shows great potential in anti AIDS and anti-virus intermediates.

With the continuous expansion of the application scope of L-ribose, the global demand for L-ribose is increasing year by year. Currently, there are chemical synthesis and biological synthesis methods for the preparation of L-ribose. However, chemical synthesis methods still have problems such as complex synthesis process routes, cumbersome reaction steps, expensive reagents, low overall yield, the need for a large amount of organic solvents, and the production of harmful by-products, which are difficult to meet the requirements of industrial production.

There are two biosynthetic methods using ribosyl alcohol as raw material and L-arabinose as raw material. Among them, the two-step conversion method using ribosyl alcohol as raw material first uses ribosyl alcohol dehydrogenase to catalyze ribosyl alcohol to produce L-ribose, and then uses L-RI enzyme to convert L-ribose to L-ribose; The production of L-arabinose as a substrate first uses L-arabinose isomerase to isomerize L-arabinose into L-ribulose, and then uses L-RI catalysis to convert L-ribulose into L-ribose.

To further reduce production costs, it is necessary to further develop immobilized enzyme catalytic processes for L-AI (L-arabinose isomerase) and MPIL-RI (L-ribose isomerase); The Haipu isomerase immobilized carrier is a biocatalyst that can be converted from a single use to multiple batches for reuse. It refers to loading bioactive enzymes onto a resin to form immobilized enzymes. Compared with free enzymes, immobilized enzymes have the advantages of high stability, easy recovery, easy separation from the reaction system, multiple reusability, and low cost.

Haipu now has multiple varieties with different skeleton structures and functional groups, which can immobilize various enzymes. It has high enzyme activity, good catalytic kinetics, high stability, and good mechanical strength, and can be widely used in the fields of food and medicine.